Full-length identity
The complete 43-residue sequence with intact N-terminal acetylation is verified by MS against the theoretical mass, distinguishing the reference material from shorter fragments.
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Compound profile
Tissue-research peptidesThymosin Beta-4 (TB-500)
Full-length, N-terminally acetylated 43-amino-acid Thymosin Beta-4 reference peptide for actin-regulation and cytoskeleton studies in vitro.
This product is full-length synthetic Thymosin Beta-4 (Tβ4): an N-terminally acetylated 43-amino-acid peptide corresponding to the widely distributed mammalian G-actin-sequestering protein. It is chemically distinct from the shorter LKKTETQ fragment that is also sometimes marketed as TB-500; the full-length and fragment materials have different mass, solubility and adsorption behavior and are not interchangeable analytical references.
Structure and class
Thymosin Beta-4 is a principal G-actin-sequestering protein: it binds monomeric actin and thereby regulates cytoskeletal dynamics in cell-free and cell-culture assays. Its biochemical interest lies in this quantifiable actin-binding stoichiometry, which makes it a reference ligand for cytoskeleton-regulation studies.
Chemistry and analytical notes
Notes cover structural and analytical chemistry; each link runs a live PubMed search for the topic.
Actin binding in vitro
Cell-free assays characterize the G-actin-sequestering interaction quantitatively; these are the primary biochemical assays in which Tβ4 serves as a reference ligand.
Search PubMedFragment distinction
LKKTETQ-class fragments behave differently in mass, solubility and surface adsorption; analytical records should state explicitly which material an experiment used.
Search PubMedSolution handling
The peptide's solubility and surface adsorption are concentration- and container-dependent, so low-binding plastics and documented preparation times improve reproducibility.
Search PubMedHandling & storage
Store lyophilized at −20°C, protected from light and moisture. Reconstitute with reconstitution-grade sterile water under aseptic technique; keep the solution at 2–8°C, avoid repeated freeze–thaw cycles and use within your protocol's stability window. For laboratory research use only — not for human or veterinary use.
For laboratory research use only
All products are sold strictly for laboratory research purposes only. Not for human or veterinary consumption, diagnostic, or therapeutic use. The information on this page is a chemistry and handling summary for qualified researchers — it is not medical advice, and nothing here constitutes a claim of safety or efficacy in humans.
Thymosin Beta-4 (TB-500)
Source this compound for your laboratory
Every batch is released only at ≥99% HPLC purity with an MS identity check, registered against a batch number you can verify online before you open the vial.