DPP-IV resistance
Substitution studies established that D-Ala at position 2 blocks DPP-IV cleavage and that further substitutions at positions 8, 15 and 27 improve solution stability — the design basis of Modified GRF (1-29).
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Compound profile
Metabolic research peptidesCJC-1295 (no DAC)
Tetrasubstituted GHRH (1-29) analog without the drug-affinity complex — a structurally simplified reference peptide for receptor-binding and enzymatic-stability studies in vitro.
CJC-1295 without DAC — also known as Modified GRF (1-29) — is an engineered analog of the shortest fully active fragment of GHRH. Native GRF (1-29) is degraded within minutes by the enzyme DPP-IV; the modified analog carries four amino-acid substitutions (including D-Ala at position 2) that protect it from this cleavage while leaving the receptor-binding core intact.
Structure and class
The peptide is a GHRH-receptor ligand in cell-based assays. Chemically, the tetrasubstitution pattern relative to GRF (1-29) confers resistance to DPP-IV and extends the practical handling window in solution — without the irreversible albumin conjugation of the DAC (Drug Affinity Complex) variant, which is why the no-DAC form is used wherever the native-scale analog is required.
Chemistry and analytical notes
Notes cover structural and analytical chemistry; each link runs a live PubMed search for the topic.
DAC versus no-DAC
The DAC variant carries a lysine-linked drug-affinity complex that binds albumin; the no-DAC analog omits it, giving a structurally simpler reference compound for receptor assays.
Search PubMedReceptor-binding assays
In-vitro GHRH-receptor binding and cAMP assays are the primary analytical contexts in which the analog is compared with native GRF (1-29).
Search PubMedSolution handling
Adsorption and solution age measurably affect assay performance, so concentration, solvent and preparation time belong in the experimental record.
Search PubMedHandling & storage
Store lyophilized at −20°C, protected from light and moisture. Reconstitute with reconstitution-grade sterile water under aseptic technique; keep the solution at 2–8°C, avoid repeated freeze–thaw cycles and use within your protocol's stability window. For laboratory research use only — not for human or veterinary use.
For laboratory research use only
All products are sold strictly for laboratory research purposes only. Not for human or veterinary consumption, diagnostic, or therapeutic use. The information on this page is a chemistry and handling summary for qualified researchers — it is not medical advice, and nothing here constitutes a claim of safety or efficacy in humans.
CJC-1295 (no DAC)
Source this compound for your laboratory
Every batch is released only at ≥99% HPLC purity with an MS identity check, registered against a batch number you can verify online before you open the vial.